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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical part in endorsing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two more splice variants had been claimed but thorough nucleotide sequence info is not really but readily available.

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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide generally consists of the epitope identified by the antibody.

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This manual outlines even further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...

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This tutorial outlines even further probable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...

Antibodies certain to the blocking peptide no more bind to your epitope about the concentrate on protein. By comparing the staining from click here your blocked antibody compared to the antibody by itself, one can see which staining is specific.

Target Information and facts Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation styles which add on the temporal coordination of each mitotic event. Cyclin E kinds a complex with and capabilities for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development via S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...

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